HGM | probeCat : Analyses and Consultancy for Scientific Projects
Molecular analyses have become the most important tool in finding the answers to questions like what, why, and how in all of the areas of life sciences. HGM Probecat offers consultancy in molecular analyses to the scientists and researchers working in life sciences. The molecular analysis work packages of your research projects will be scientifically evaluated, and will be designed with all of its scientific, technical and financial aspects.

Microarray Analyses

The analysis of data obtained during the last decade with the accomplishment of genome projects has become possible with the advent of high-throughput analysis technologies. The most well known of these technologies is microarray analysis.

  • Transcriptome analysis: By comparing the gene expression profile of cells and tissues through microarray analysis, it is now possible to evaluate the effects of a specific agent or different diseases on transcriptome. Besides the analysis of physiological and metabolic pathways transcriptome analysis may lead to the identification of molecular signatures specific to diseases and new target molecules for drugs.
  • Genome analysis: High resolution genome-wide SNP microarrays can be used for:
    • The identification of susceptibility genes for multigenic and multifactorial diseases through association analysis.
    • The identification of genes in Mendelian disorders through linkage analysis.
    • The identification of copy number changes with much higher resolution compared to classical cytogenetic analysis.

 

See our microarray bioinformatics tools

Next Generation Sequencing Approaches

In the era of genome projects at the last point where the omics technologies reached next generation sequencing technologies that can accomplish genome projects with a single instrument, to much less cost compared to classical sequencing technologies, were started to be developed. These technologies are still under development. Today with current technology it is possible to read 500 MB in seven hours run.

Next generation sequencing technologies not only can be used for de novo genome sequencing but they also have a large perspective of applications.

  • De Novo Sequencing: To sequence the genome of an organism that is not sequenced before
  • Resequencing: To sequence genome of different individuals of an organism that is sequenced before.
  • Targeted sequencing: A genomic region up to 5 MB captured with specially designed microarrays can be sequenced through next generation technologies.
  • Amplicon sequencing: Genes in different loci or a gene which is dispersed to a large genomic region can be sequenced in several different samples all in parallel.
  • Deep Amplicon Sequencing: When the cells composing the sample is heterogenous (such as tumour samples) the differences of the genomes of different cells can be captured (1% of the cells) with next generation sequencing.
  • Methylation analysis: Determination of the methylation of level of genes of interest.
  • Metagenomics